Dibutyl Phthalate Exposure Disrupts Evolutionarily Conserved Insulin and Glucagon-Like Signaling in Drosophila Males | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

3350270

Reference Type

Journal Article

Title

Dibutyl Phthalate Exposure Disrupts Evolutionarily Conserved Insulin and Glucagon-Like Signaling in Drosophila Males

Author(s)

Williams, MJ; Wiemerslage, L; Gohel, P; Kheder, S; Kothegala, LV; Schioth, HB

Year

2016

Is Peer Reviewed?

Yes

Journal

Endocrinology
ISSN: 0013-7227
EISSN: 1945-7170

Volume

157

Issue

Page Numbers

2309-2321

Language

English

PMID

27100621

DOI

10.1210/en.2015-2006

Web of Science Id

WOS:000377214600012

Abstract

Phthalate diesters are commonly used as industrial plasticisers, as well as in cosmetics and skin care products, as a result people are constantly exposed to these xenobiotics. Recent epidemiological studies have found a correlation between circulating phthalate levels and type 2 diabetes, whereas animal studies indicate that phthalates are capable of disrupting endocrine signaling. Nonetheless, how phthalates interfere with metabolic function is still unclear. Here, we show that feeding Drosophila males the xenobiotic dibutyl phthalate (DBP) affects conserved insulin- and glucagon-like signaling. We report that raising flies on food containing DBP leads to starvation resistance, increased lipid storage, hyperglycemia, and hyperphagia. We go on to show that the starvation-resistance phenotype can be rescued by overexpression of the glucagon analogue adipokinetic hormone (Akh). Furthermore, although acute DBP exposure in adult flies is able to affect insulin levels, only chronic feeding influences Akh expression. We establish that raising flies on DBP-containing food or feeding adults DBP food affects the expression of homologous genes involved in xenobiotic and lipid metabolism (AHR [Drosophila ss], NR1I2 [Hr96], ABCB1 [MDR50], ABCC3 [MRP], and CYP3A4 [Cyp9f2]). Finally, we determined that the expression of these genes is also influenced by Akh. Our results provide comprehensive evidence that DBP can disrupt metabolism in Drosophila males, by regulating genes involved in glucose, lipid, and xenobiotic metabolism.

Keywords

ATP Binding Cassette Transporter, Subfamily B/metabolism; Animals; Cytochrome P-450 CYP3A/metabolism; Dibutyl Phthalate/toxicity; Drosophila; Drosophila Proteins/metabolism; Environmental Pollutants/toxicity; Glucagon/metabolism; Insulin/metabolism; Lipid Metabolism/drug effects; Signal Transduction/drug effects; Xenobiotics/metabolism; 2286E5R2KE; 9007-92-5