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HERO ID
3859094
Reference Type
Journal Article
Title
Degradation of di(2-Ethylhexyl) phthalate by a novel Gordonia alkanivorans strain YC-RL2
Author(s)
Nahurira, R; Ren, L; Song, J; Jia, Y; Wang, J; Fan, S; Wang, H; Yan, Y
Year
2017
Is Peer Reviewed?
Yes
Journal
Current Microbiology
ISSN:
0343-8651
EISSN:
1432-0991
Volume
74
Issue
3
Page Numbers
309-319
Language
English
PMID
28078431
DOI
10.1007/s00284-016-1159-9
Web of Science Id
WOS:000395129800002
URL
https://search.proquest.com/docview/2000549896?accountid=171501
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Abstract
One bacterial strain, YC-RL2, isolated from petroleum-contaminated soil, could utilize environmental hormone Di(2-Ethylhexyl) phthalate (DEHP) as a sole carbon source for growth. Strain YC-RL2 was identified as Gordonia alkanivorans by 16S rRNA gene analysis and Biolog tests. The effects of environmental factors which might affect the degrading process were optimized at 30 °C and pH 8.0. Strain YC-RL2 showed superior halotolerance and could tolerate up to 0-5% NaCl in trace element medium supplemented with DEHP, although the DEHP degradation rates slowed as NaCl concentration increased. It also showed an outstanding performance in a wide range of pH (6.0-11.0). Meanwhile, strain YC-RL2 was able to withstand high concentrations of DEHP (from 100 to 800 mg/L), and the degradation rates were all above 94%. The DEHP intermediates were detected by HPLC-MS, and the degradation pathway was deduced tentatively. DEHP was transformed into phthalic acid (PA) via mono (2-ethylhexyl) phthalate (MEHP), and PA was further utilized for growth via benzoic acid (BA). The enzyme expected to catalyze the hydrolysis of MEHP to PA was identified from strain YC-RL2. Further investigation found that the enzyme could catalyze the transformation of a wide range of monoalkyl phthalates to PA. This study is the first report about species G. alkanivorans which could degrade several kinds of phthalic acid esters (PAEs), and indicates its application potential for bioremediation of PAE-polluted sites.
Keywords
article; benzoic acid; bioremediation; environmental factors; high performance liquid chromatography; hydrolysis; mass spectrometry; phthalates; phthalic acid; ribosomal RNA; salt tolerance; sodium chloride; trace elements
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jan 2017 - July 2017
WOS
LitSearch July 2017 - Sept 2018
Prior Search Overlap
WOS
•
Diethyl phthalate (DEP)
Database searches
Jul 2017 update
Web of Science
Jan 2020 update
Web of Science
New for this project
Excluded: No Primary Data on Health Effects
Not chemical specific
Chemical treatment/disposal/remediation
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