US EPA

2859388 

Journal Article 

Batch and fed-batch bioreactor cultivations of a Thermus species with thermophilic BTEX-degrading activity 

Chen C-I; Taylor, RT 

1997 

Yes 

Applied Microbiology and Biotechnology
ISSN: 0175-7598
EISSN: 1432-0614 

BIOSIS/97/22887 

47 

6 

726-733 

English 

10.1007/s002530051002 

WOS:A1997XK88200018 

https://www.proquest.com/scholarly-journals/batch-fed-bioreactor-cultivations-thermus-species/docview/884655707/se-2?accountid=171501
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BIOSIS COPYRIGHT: BIOL ABS. The thermophilic bacterium, Thermus species ATCC 27978, which is capable of aerobically degrading benzene, toluene, ethylbenzene, and the xylenes (BTEX), was cultured in 5-1 fermentors on a Castenholz salts-tryptone medium. This bacterium can be cultivated more conveniently at 45� C, a temperature substantially lower than its optimal growth temperature (approx. 60� C). Yet, the washed harvested cells from such cultures display the same initial BTEX-degrading activity as those when Thermus sp. is grown at its higher optimal temperature. Two bioreactor cultivation modes, batch and fed batch, were investigated. More biomass and more BTEX-degrading activity (assayed at 60� C) were generated in fed-batch cultures than in the growth-limited batch cultures. The former yielded a biomass concentration of 2.5 g dry cell weight (DCW) l-1 and whole-cell degrading specific activities of 7.6 | 1.3, 10.1 | 1.9, 9.8 | 2.1, 2.3 | 0.5, and 4.6 | 0.9 nmol degraded (mg DCW)-1 min-1 for benz 

Biology--Biotechnology; Fed-batch culture; Thermophile; Microbiology; Bacteria; Temperature; Growth rate; Hydrocarbons