US EPA

1326381 

Journal Article 

Atypical Leydig cell hyperplasia in adult rats with low T and high LH induced by prenatal di(n-butyl) phthalate exposure 

Wakui, S; Takahashi, H; Mutou, T; Shirai, M; Jutabha, P; Anzai, N; Wempe, MF; Kansaku, N; Hano, H; Inomata, T; Endou, H 

2013 

Yes 

Toxicologic Pathology
ISSN: 0192-6233
EISSN: 1533-1601 

Toxicol Pathol. 2013; 41(3):480-6. [Toxicologic pathology] 

41 

3 

480-486 

English 

22968287 

10.1177/0192623312457272 

WOS:000331464200005 

https://search.proquest.com/docview/1348486369?accountid=171501
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The present study describes atypical Leydig cell (LC) hyperplasia in 20-week-old Sprague-Dawley rats with low testosterone and high luteinizing hormone levels after prenatal administration of 100 mg/kg/day di(n-butyl) phthalate on days 12 to 21 postconception. Light microscopy revealed LC hyperplasia surrounded by severely degenerated seminiferous tubules. Aggregated LCs had large ovoid nuclei with nucleoli and abundant eosinophilic cytoplasm. Immunohistochemical analysis showed expression of proliferating cell nuclear antigen and vimentin in many hyperplastic LCs. Electron microscopy revealed atypical nuclei, abundant free ribosomes, stripped rough endoplasmic reticulum, intermediate-size filaments, elongated cytoplasmic filopodia, atypical tight junctions, and cilia formations, but smooth endoplasmic reticulum was scarcely observed. 

Toxicology Abstracts; electron microscopy; atypical Leydig cell; hyperplasia; prenatal DBP exposure; Vimentin; Phthalic acid; Tight junctions; Leukocytes (eosinophilic); Ribosomes; Pseudopodia; Nucleoli; Testosterone; Filaments; Leydig cells; Proliferating cell nuclear antigen; Seminiferous tubule; Prenatal experience; Endoplasmic reticulum; Cytoplasm; X 24350:Industrial Chemicals