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Citation
Tags
HERO ID
1337530
Reference Type
Journal Article
Title
Molecular identification of an arsenic four-gene operon in Campylobacter lari
Author(s)
Nakajima, T; Hayashi, K; Nagatomi, R; Matsubara, K; Moore, JE; Millar, BC; Matsuda, MM
Year
2013
Is Peer Reviewed?
Yes
Journal
Folia Microbiologica
ISSN:
0015-5632
EISSN:
1874-9356
Volume
58
Issue
3
Page Numbers
253-260
Language
English
PMID
23132657
DOI
10.1007/s12223-012-0207-5
Web of Science Id
WOS:000317976600011
URL
http://link.springer.com/10.1007/s12223-012-0207-5
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Abstract
An arsenic (ars) four-gene operon, containing genes encoding a putative membrane permease (ArsP), a transcriptional repressor (ArsR), an arsenate reductase (ArsC) and an arsenical-resistance membrane transporter (Acr3) was first identified in urease-positive thermophilic Campylobacter (UPTC) isolate, CF89-12. UPTC CF89-12 and some other Campylobacter lari isolates contained their ars four-genes, similarly, differing from that in the reference C. lari RM2100 strain. Two putative promoters and a putative terminator were identified for the operon in UPTC CF89-12. In vivo transcription of the operon was confirmed in the UPTC cells. PCR experiments using two primer pairs designed in silico to amplify two arsR and arsC-acr3 segments, respectively, generated two amplicons, approximately 200 and 350 base pairs, with all 31 of 31 and 19 of 31 C. lari isolates (n = 17 for UPTC; n = 14 for UN C. lari), respectively. An inverted repeat forming a dyad structure, a potential binding site for a transcriptional repressor, was identified in the promoter region. Within the deduced 61 amino acids sequence of the putative arsR open reading frame from the UPTC CF89-12, a metal binding box and a DNA-binding helix-turn-helix motif were identified. The UPTC CF89-12 and some other UPTC isolates isolated from natural environment were resistant to arsenate.
Tags
IRIS
•
Arsenic Hazard ID
1. Initial Lit Search
PubMed
PubMed
Considered New
PubMed
WOS
Considered New
2. Lit Search Updates through Oct 2015
PubMed
WOS
Considered
4. Considered through Oct 2015
7. Other Studies through Oct 2015
Other
•
Arsenic (Inorganic)
1. Literature
PubMed
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
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