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HERO ID
1597641
Reference Type
Journal Article
Title
Rapid and sensitive analysis of phthalate metabolites, bisphenol A, and endogenous steroid hormones in human urine by mixed-mode solid-phase extraction, dansylation, and ultra-performance liquid chromatography coupled with triple quadrupole mass spectrometry
Author(s)
Wang, HX; Wang, B; Zhou, Y; Jiang, QW
Year
2013
Is Peer Reviewed?
Yes
Journal
Analytical and Bioanalytical Chemistry
ISSN:
1618-2642
EISSN:
1618-2650
Volume
405
Issue
12
Page Numbers
4313-4319
Language
English
PMID
23430180
DOI
10.1007/s00216-013-6779-3
Web of Science Id
WOS:000317643900043
Abstract
Steroid hormone levels in human urine are convenient and sensitive indicators for the impact of phthalates and/or bisphenol A (BPA) exposure on the human steroid hormone endocrine system. In this study, a rapid and sensitive method for determination of 14 phthalate metabolites, BPA, and ten endogenous steroid hormones in urine was developed and validated on the basis of ultra-performance liquid chromatography coupled with electrospray ionization triple quadrupole mass spectrometry. The optimized mixed-mode solid phase-extraction separated the weakly acidic or neutral BPA and steroid hormones from acidic phthalate metabolites in urine: the former were determined in positive ion mode with a methanol/water mobile phase containing 10 mM ammonium formate; the latter were determined in negative ion mode with a acetonitrile/water mobile phase containing 0.1 % acetic acid, which significantly alleviated matrix effects for the analysis of BPA and steroid hormones. Dansylation of estrogens and BPA realized simultaneous and sensitive analysis of the endogenous steroid hormones and BPA in a single chromatographic run. The limits of detection were less than 0.84 ng/mL for phthalate metabolites and less than 0.22 ng/mL for endogenous steroid hormones and BPA. This proposed method had satisfactory precision and accuracy, and was successfully applied to the analyses of human urine samples. This method could be valuable when investigating the associations among endocrine-disrupting chemicals, endogenous steroid hormones, and relevant adverse outcomes in epidemiological studies.
Keywords
Phthalate metabolites; Bisphenol A; Endogenous steroid hormones; Mixed-mode solid-phase extraction; Liquid chromatography-tandem mass spectrometry; Urine
Tags
IRIS
•
Formaldehyde
Exposure Litsearch Jan 2012 - Aug 2015
PubMed
Human exposure to formaldehyde
Exposure levels, formaldehyde
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
WOS
Toxnet
Excluded
Source – no date limit through June 2013 (Private)
Pubmed
Source - Dec 2013 Update (Private)
WOS
ToxNet
Source - Jun 2014 Update (Private)
WOS
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