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Citation
Tags
HERO ID
1677619
Reference Type
Journal Article
Title
Characterization of arsenate uptake by cultured primary rat astrocytes
Author(s)
Koehler, Y; Dringen, R
Year
2013
Is Peer Reviewed?
1
Journal
Neurochemical Research
ISSN:
0364-3190
EISSN:
1573-6903
Volume
38
Issue
9
Page Numbers
1785-1790
Language
English
PMID
23729299
DOI
10.1007/s11064-013-1081-5
Web of Science Id
WOS:000322722700004
URL
http://link.springer.com/10.1007/s11064-013-1081-5
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Abstract
Arsenate is known to be accumulated by cultured astrocytes and to stimulate astrocytic glutathione export, but the arsenate uptake into astrocytes has not been characterized so far. To address this topic, we have exposed primary rat astrocyte cultures to arsenate and determined the cellular arsenic content by atomic absorption spectroscopy. Viable astrocytes accumulated arsenate in a time- and concentration-dependent manner. Their cellular arsenic content increased almost proportional with time for up to 60 min after application of arsenate. Analysis of the concentration-dependent increase in the specific arsenic content of the cells after 30 min of arsenate exposure revealed that cultured astrocytes take up arsenate with saturable kinetics by a transport process that has apparent KM- and Vmax-values of 1.7 ± 0.2 mM and 28 ± 4 nmol/(mg protein × 30 min), respectively. Arsenate uptake in viable astrocytes was strongly inhibited by the presence of phosphate or by lowering the incubation temperature to 4 °C and was completely abolished in a sodium ion-free medium. These results strongly suggest that the saturable temperature-dependent arsenate uptake into astrocytes is mediated by a sodium-dependent phosphate cotransporter.
Keywords
Arsenic; Astroglia; Phosphate transporter; Uptake
Tags
IRIS
•
Arsenic Hazard ID
PubMed
Considered New
PubMed
Considered New
PubMed
WOS
Considered New
2. Lit Search Updates through Oct 2015
PubMed
WOS
Considered
7. Other Studies through Oct 2015
PBPK/TK
•
Arsenic (Inorganic)
1. Literature
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
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