US EPA

1936033 

Journal Article 

Novel identification of lipid-soluble arsenic compounds using chemical derivatisations in conjunction with RP-HPLC-ICPMS/ESMS 

Amayo, KO; Raab, A; Krupp, EM; Gunnlaugsdottir, H; Feldmann, J 

2013 

Yes 

Analytical Chemistry
ISSN: 0003-2700
EISSN: 1520-6882 

85 

19 

9321–9327 

English 

23984920 

10.1021/ac4020935 

The identification of molecular structures of arsenolipid is pivotal for its toxicological assessment and in understanding the arsenic cycling in the environment. However, analysis of these compounds in lipid matrix is an ongoing challenge. So far only few arsenolipids have been reported, such as arsenic fatty acids (AsFAs) and arsenic hydrocarbons (AsHCs). By means of RP-HPLC-ICPMS/ESMS, we investigated Capelin oil (Mallotus villosus) for possible new species of arsenolipid. Twelve arsenolipids were identified in the fish oil including three AsFAs and seven AsHCs. Among the AsHCs, four of them identified with protonotated molecular masses 305, 331, 347 and 359 have not been reported before. In addition, the compounds with molecular formulae C20H44AsO+ and C24H44AsO+ found in low concentrations showed chromatographic properties and MS data consistent with cationic trimethylarsenio fatty alcohols. Derivatisation by acetylation and thiolation coupled with accurate mass spectrometry was successfully used to establish the occurrence of this new class of arsenolipids as cationic trimethylarsenio fatty alcohols (TMAsFOH).