US EPA

2510761 

Journal Article 

Nuclear factor erythroid 2-related factor 2 rescues the oxidative stress induced by di-N-butylphthalate in testicular Leydig cells 

Shen, B; Wang, W; Ding, L; Sao, Y; Huang, Y; Shen, Z; Zhuo, Y; Wei, Z; Zhang, W 

2015 

Yes 

Human & Experimental Toxicology
ISSN: 0960-3271
EISSN: 1477-0903 

Hum Exp Toxicol. 

34 

2 

145-152 

English 

24917652 

10.1177/0960327114530744 

WOS:000349087600004 

https://search.proquest.com/docview/1652443956?accountid=171501
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AIM: This study aimed to determine whether nuclear factor erythroid 2-related factor 2 antagonized the oxidative stress induced by di-N-butylphthalate (DBP) in testicular Leydig cells.

METHODS: Mouse TM3 testicular Leydig cells were treated with Nrf2 knockdown (KD) or overexpression in the presence and absence of DBP. Oxidative profiles were examined. Nrf2 target antioxidant genes were studied, and the effects of Nrf2 inducer sulphoraphane (SFN) were tested.

RESULTS: DBP induced intracellular oxidative stress to a similar extent with Nrf2 KD. Expression and protein levels of Nrf2 were increased together with its target genes, namely heme oxygenase 1, nicotinamide adenine dinucleotide phosphate quinone oxidoreductase 1 and peroxiredoxin 6, following DBP stimulation. Use of SFN not only restored the intracellular oxidative toxicity but also cell proliferation and testosterone secretion in response to DBP.

CONCLUSION: Increased Nrf2 activity, for example, by SFN can effectively antagonize the oxidative stress in testicular Leydig cells caused by DBP. 

Di-N-butylphthalate; Nrf2; testicular Leydig cells; oxidative stress