Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
2858129
Reference Type
Journal Article
Title
A new organic solvent tolerant protease from Bacillus pumilus 115b
Author(s)
Abd Rahman, R; Mahamad, S; Salleh, A; Basri, M
Year
2007
Is Peer Reviewed?
Yes
Journal
Journal of Industrial Microbiology and Biotechnology
ISSN:
1367-5435
EISSN:
1476-5535
Volume
34
Issue
7
Page Numbers
509-517
Language
English
PMID
17492323
DOI
10.1007/s10295-007-0222-8
Web of Science Id
WOS:000247242400006
Abstract
Five out of the nine benzene-toulene-ethylbenzene-xylene (BTEX) tolerant bacteria that demonstrated high protease activity on skim milk agar were isolated. Among them, isolate 115b identified as Bacillus pumilus exhibited the highest protease production. The protease produced was stable in 25% (v/v) benzene and toluene and it was activated 1.7 and 2.5- fold by n-dodecane and n-tetradecane, respectively. The gene encoding the organic solvent tolerant protease was cloned and its nucleotide sequence determined. Sequence analysis revealed an open reading frame (ORF) of 1,149 bp that encoded a polypeptide of 383 amino acid residues. The polypeptide composed of 29 residues of signal peptide, a propeptide of 79 residues and a mature protein of 275 amino acids with a calculated molecular mass of 27,846 Da. This is the only report available to date on organic solvent tolerant protease from B. pumilus.
Keywords
organic solvent tolerant protease; cloning; Bacillus pumilus; isolation
Tags
•
Ethylbenzene
Database searches
WOS
LitSearch Update 2019
WOS
New Litsearch 4/2019
PubMed
Pre-2020 Screening HERO tags
Data set for title/abstract screening
Excluded – PECO criteria not met
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity