US EPA

3858470 

Journal Article 

High-flux Detection of 30 Kinds Amino Acids with Isotope-Labeled Internal Standard by LC-MS/MS 

Li Peng-Fei; Tao Bei-Bei; Zhang Xu-De; An Zhuo-Ling; Zhang Xi; Li Yue; Hao Qian-Qian; Liu Li-Hong 

2013 

Yes 

Chinese Journal of Analytical Chemistry / Fen Xi Hua Xue
ISSN: 0253-3820 

41 

9 

1347-1352 

10.3724/SP.J.1096.2013.30076 

WOS:000329520400006 

A High-flux LC-MS/MS method has been developed for simultaneous detection of 30 amino acids by stable isotope labeled. The biological samples were added with methanol solution containing 12 kinds of isotope. labeled amino acids as internal standard, and then derivatization reagents were added after precipitated and dried by nitrogen. The amino acids and isotope. labeled amino acids were derived together to reduce the systematic error. The amino acids were separated on a TC-C-18 column using water and acetonitrile(both containning 0. 01% heptafluorobutyric acid and 0. 1% formic acid) as mobile phase by gradient elution. 30 kinds of amino acids and isomers could be separated in chromatographic baseline. Detection was carried out by multiple reaction monitoring(MRM) on a 3200QTRAP LC-MS/MS system. Real time monitoring for patients with malignant blood disease patterns of plasma amino acids can directly reflect the body's metabolism and nutritional status. It can improve the success rate of transplantation patients and the life quality of patients after transplantation. 

Liquid chromatography tandem mass spectrometry; Amino acid; High-flux quantitating; Isotope internal standard; Malignant blood disease