Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1579288
Reference Type
Journal Article
Title
Biosorption of heavy metals by lactic acid bacteria and identification of mercury binding protein
Author(s)
Kinoshita, H; Sohma, Y; Ohtake, F; Ishida, M; Kawai, Y; Kitazawa, H; Saito, T; Kimura, K
Year
2013
Is Peer Reviewed?
1
Journal
Research in Microbiology
ISSN:
0923-2508
EISSN:
1769-7123
Publisher
ELSEVIER
Location
AMSTERDAM
Volume
164
Issue
7
Page Numbers
701-709
Language
English
PMID
23603782
DOI
10.1016/j.resmic.2013.04.004
Web of Science Id
WOS:000324010800003
Abstract
Heavy metals cause various health hazards. Using lactic acid bacteria (LAB), we tested the biosorption of heavy metals e.g. cadmium (Cd) (II), lead (Pb) (II), arsenic (As) (III), and mercury (Hg) (II). Cd (II) sorption was tested in 103 strains using atomic absorption spectrophotometery (AAS). Weissella viridescens MYU 205 (1×10(8) cells/ml) decreased Cd (II) levels in citrate buffer (pH 6.0) from one ppm to 0.459 ± 0.016 ppm, corresponding to 10.46 μg of Cd (II). After screening, 11 LAB strains were tested using various pH (pH 4.0, 5.0, 6.0, 7.0) showing the sorption was acid sensitive; and was cell concentration dependent, where the Cd (II) concentration decreased from one ppm to 0.042 (max) / 0.255 (min) ppm at 1×10(10) cells/ml. Additionally, the biosorption of Pb (II), As (III), and Hg (II) were tested using an inductively coupled plasma mass spectrometer (ICP-MS). The Hg (II) concentration was reduced the most followed by Pb (II) and As (III). Many of the bacterial cell surface proteins of W. viridescens MYU 205 showed binding to Hg (II) using the Hg (II) column assay. Having a CXXC motif, a ∼14 kDa protein may be one of the Hg (II) binding proteins. LAB biosorption may aid the detoxification of people exposed to heavy metals.
Keywords
Heavy metal; Biosorption; Lactic acid bacteria; Detoxification; Cadmium; Mercury
Tags
IRIS
•
Arsenic Hazard ID
PubMed
Considered New
PubMed
Considered New
PubMed
WOS
Considered New
2. Lit Search Updates through Oct 2015
PubMed
WOS
Considered
7. Other Studies through Oct 2015
Other
•
Arsenic (Inorganic)
1. Literature
Lit search updates through Oct 2015
3. Hazard ID Screening
Other potentially supporting studies
•
Methylmercury
ADME Search: Jan 1990 - Nov 2018
Results with mercury
PubMed
WoS
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity