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2851110 
Journal Article 
[The application of UPLC-MS/MS to detect precursors of perfluorooctanoic acid and perfluoroalkyl carboxylatesin serum] 
Shi, Y; Yang, L; Li, J; Zhao, Y; Wu, Y 
2014 
Zhonghua Yufang Yixue Zazhi / Chinese Journal of Preventive Medicine
ISSN: 0253-9624 
48 
628-633 
Chinese 
Objective: We established a method of UPLC-MS/MS that was to detect fifteen precursors of perfluoroalkyl sulfonates (PFSA) and perfluoroalkyl carboxylates (PFCA) in serum.

Methods: Briefly, TBAS solution was added to sera, then the mixed solution was extracted with aliquots of MTBE. The MTBE aliquots were combined, evaporated to dryness under nitrogen, and reconsituted in 0.25 ml of methanol and water (1:1). Then the reconstituted solution through 0.2 µm nylon syringe filter was collected. Chromatographic separation was performed using a Waters ACQUITY (TM) BEH ¹⁸C column (50 mm × 2.1 mm × 1.7 mm). Analyte quantitation was performed in the negative electrospray ionization mode and multiple reaction monitoring (MRM).

Results: Three target substances, 6: 6PFPi, 6: 8PFPi, 8: 8PFPi, were externally confirmed by standard addition. Rates of recovery for these three chemicals were from 41.01% to 112.13% in two standard levels. And the relative standard deviations (RSD) were lower than 11.63% and higher than 1.80%. The other twelve substances were quantified with internal standard. Moreover in two standard levels, rate of recovery for these chemicals ranged from 70.25% to 127.51%. And RSD were more than 1.23% and less than 15.45%. And the corresponding limit of detection (LOD) and limit of quantitation (LOQ) for all target substances were 0.1-5.0 pg/ml and 0.2-10.0 pg/ml. Then we detected these target substances in ten different human serum samples. The levels of few substances were higher than LOD. And the ranges of FOSA-M, N-EtFOSA-M, N-MeFOSAA, N-EtFOSAA were respectively < LOD-0.94 pg/ml, < LOD-10.08 pg/ml, < LOD-6.74 pg/ml, < LOD-1.04 pg/ml.

Conclusion: The method, with high sensitivity and accuracy, could meet the actual testing requirements. 
chromatography; liquid; tandem mass spectrometry; serum 
PFAS
• Additional PFAS (formerly XAgency)
     Literature Search November 2019
          PubMed
     2-(N-Methylperfluorooctanesulfonamido)acetic acid
• Expanded PFAS SEM (formerly PFAS 430)
     Litsearch: September 2019
          PubMed
     Not prioritized for screening
     Potassium perfluorooctanoate
     Sodium perfluorooctanoate
     2-(N-Ethyl-perfluorooctanesulfonamido)acetate
     2-(N-Ethylperfluorooctanesulfonamido)acetic acid
     2-(N-Methylperfluorooctanesulfonamido)acetic acid
• NMeFOSAA
     Literature Search
          Pubmed
     Screening Results
          Excluded/Not on Topic
• ^Per- and Polyfluoroalkyl Substances (PFAS)
     PFOA (335-67-1) and PFOS (1763-23-1)
          Literature Search – Adverse outcome pathway (2015-present)
               Pubmed
     NMeFOSAA (2355-31-9)
          Literature Search
               Pubmed
• PFAS 150
     Literature Search Update December 2020
          PubMed
     Literature Search August 2019
          PubMed
     Ammonium perfluorooctanoate
     N-Ethylperfluorooctanesulfonamide
     Perfluorooctanoic acid
• PFAS Universe
     Data Source
          Pubmed
     2-(N-Ethyl-perfluorooctanesulfonamido)acetate
     2-(N-Ethylperfluorooctanesulfonamido)acetic acid
     2-(N-Methylperfluorooctanesulfonamido)acetic acid
     N-Ethylperfluorooctanesulfonamide
     Perfluorooctanesulfonamide
• PFOSA
     Literature Search
          Pubmed
     Screening Results
          Excluded/Not on Topic
OW - HHRAB
• PFOA (335-67-1) and PFOS (1763-23-1)
     Literature Search – Adverse outcome pathway (2015-present)
          Pubmed
     Screening Results
          Excluded/Not on Topic
     Literature Search Update (2013-2019)
          PubMed