Determination of mandelic and phenylglyoxylic acids in rat urine by high-performance liquid chromatography and by isotachophoresis | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

2859027

Reference Type

Journal Article

Title

Determination of mandelic and phenylglyoxylic acids in rat urine by high-performance liquid chromatography and by isotachophoresis

Author(s)

Sollenberg, J; Smallwood, AW; Lowry, LK

Year

1985

Is Peer Reviewed?

Journal

Journal of Chromatography
ISSN: 0021-9673

Volume

343

Issue

Page Numbers

175-178

Language

English

PMID

4066851

DOI

10.1016/S0378-4347(00)84582-X

Web of Science Id

WOS:A1985ARY5600021

Abstract

The metabolism of ethylbenzene in the rat has been well characterized following inhalation exposure [l] . The major metabolites have been identified as l-phenylethanol, o
hydroxyacetophenone, mandelic acid (MA), phenylglyoxylic acid (PGA), hippuric acid, and phenylaceturic acid [Z]. In humans, the major urinary metabolites have been identified as MA and PGA, and these metabolites have been suggested for monitoring occupational exposure to ethylbenzene [3, 41. Several methods have been used to determine MA and PGA in urine including gas chromatography (GC) [ 51, high-performance liquid chromatography (HPLC) [6] , and isotachophoresis (ITP) [ 71. The purpose of this study was to evaluate HPLC and ITP methods for the analysis of MA and PGA in rat urine as a part of a biotransformation study of ethylbenzene.