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HERO ID
3981273
Reference Type
Journal Article
Title
Coupling sweeping-micellar electrokinetic chromatography with tandem mass spectrometry for the therapeutic monitoring of benzimidazoles in animal urine by dilute and shoot
Author(s)
Tejada-Casado, C; Moreno-González, D; Del Olmo-Iruela, M; García-Campaña, AM; Lara, FJ
Year
2017
Is Peer Reviewed?
1
Journal
Talanta
ISSN:
0039-9140
EISSN:
1873-3573
Publisher
ELSEVIER SCIENCE BV
Location
AMSTERDAM
Volume
175
Page Numbers
542-549
Language
English
PMID
28842031
DOI
10.1016/j.talanta.2017.07.080
Web of Science Id
WOS:000412033100073
URL
https://linkinghub.elsevier.com/retrieve/pii/S0039914017308020
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Abstract
A new method based on micellar electrokinetic chromatography-tandem mass spectrometry (MEKC-MS/MS) has been developed for the identification and simultaneous quantification of thirteen benzimidazoles in animal urine. In order to obtain an appropriate separation with the highest sensitivity, different electrophoretic parameters were evaluated. Under optimum conditions, the separation was performed using ammonium perfluorooctanoate as volatile surfactant and electrophoretic buffer (50mM, pH 9). To increase the sensitivity, a stacking mode named sweeping was applied, using water as injection solvent at 50mbar for 75s, obtaining sensitivity enhancement factors from 50 to 181. The method was applied to different animal urine samples, including sheep, cow and goat. The sample treatment consisted of a 1:10 (v/v) dilution with water. Calibration using sheep urine samples can be used for both goat and cow urine samples with a relative bias below 25% and relative standard deviations lower than 8%. The limits of detection were below 70µgL(-1). As a result, the applicability of this rapid, simple, sensitive, and environmentally friendly method for therapeutic drug monitoring of benzimidazoles based on the analysis of animal urine has been demonstrated.
Keywords
Animal urine; Benzimidazoles; Micellar electrokinetic chromatography; Sweeping; Tandem mass spectrometry; Dilute and shoot
Tags
PFAS
•
Additional PFAS (formerly XAgency)
•
Expanded PFAS SEM (formerly PFAS 430)
Litsearch: September 2019
PubMed
Not prioritized for screening
Potassium perfluorooctanoate
Sodium perfluorooctanoate
•
^Per- and Polyfluoroalkyl Substances (PFAS)
PFOA (335-67-1) and PFOS (1763-23-1)
Literature Search – Adverse outcome pathway (2015-present)
Pubmed
•
PFAS 150
Literature Search August 2019
PubMed
Web of Science
Not prioritized for screening
Ammonium perfluorooctanoate
Perfluorooctanoic acid
•
PFOA (335-67-1) and PFOS (1763-23-1)
Literature Search – Adverse outcome pathway (2015-present)
Pubmed
Screening Results
Excluded/Not on Topic
Literature Search Update (2013-2019)
PubMed
WOS
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