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3230369 
Journal Article 
Study on the mechanism of action between dimethyl phthalate and herring sperm DNA at molecular level 
Chi, Z; Wang, D; You, H 
2016 
Yes 
Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes
ISSN: 0360-1234
EISSN: 1532-4109 
51 
553-557 
English 
Dimethyl phthalate (DMP), a typical phthalic acid ester, is widespread in the environment and causes extensive concern due to its adverse effects on human health. To understand the genotoxicity of DMP at molecular level, the toxic interaction of DMP with herring sperm (hs) deoxyribonucleic acid (DNA; hs-DNA) was investigated in vitro under simulated physiological conditions using multi-spectroscopic techniques and a molecular modeling method. The results of Ultraviolet-Visible absorption spectroscopy, fluorescence emission spectroscopy, and circular dichroism spectra indicated that DMP interacts with hs-DNA in a groove-binding mode that changes the double helical structure of DNA. The binding constant and the number of binding sites calculated from the fluorescence quenching data were 565.718 L mol(-1) and 0.7872, respectively. A molecular modeling study revealed that DMP tends to bind with DNA in the A-T-rich regions of minor groove and that hydrogen bonding and van der Waals forces play main roles in the interaction. This research can help to elucidate the mechanism of DMP toxicity in vivo. 
IRIS
• Dibutyl Phthalate (DBP)
     Database Searches
          Litsearch Jan 2016 - July 2016
               Pubmed
     Excluded: No Primary Data on Health Effects
          Not chemical specific
• Phthalates – Targeted Search for Epidemiological Studies
     Source – all searches
          Pubmed
          WOS
     Excluded
     Source - Jun 2016 Update (Private)
          Pubmed
     Source - Dec 2016 Update (Private)
          Pubmed
          WOS