Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
3230369
Reference Type
Journal Article
Title
Study on the mechanism of action between dimethyl phthalate and herring sperm DNA at molecular level
Author(s)
Chi, Z; Wang, D; You, H
Year
2016
Is Peer Reviewed?
Yes
Journal
Journal of Environmental Science and Health, Part B: Pesticides, Food Contaminants, and Agricultural Wastes
ISSN:
0360-1234
EISSN:
1532-4109
Publisher
Taylor & Francis Ltd.
Location
Philadelphia
Volume
51
Issue
8
Page Numbers
553-557
Language
English
PMID
27166703
DOI
10.1080/03601234.2016.1170557
Web of Science Id
WOS:000378341000008
URL
https://search.proquest.com/docview/1795879611?accountid=171501
Exit
Abstract
Dimethyl phthalate (DMP), a typical phthalic acid ester, is widespread in the environment and causes extensive concern due to its adverse effects on human health. To understand the genotoxicity of DMP at molecular level, the toxic interaction of DMP with herring sperm (hs) deoxyribonucleic acid (DNA; hs-DNA) was investigated in vitro under simulated physiological conditions using multi-spectroscopic techniques and a molecular modeling method. The results of Ultraviolet-Visible absorption spectroscopy, fluorescence emission spectroscopy, and circular dichroism spectra indicated that DMP interacts with hs-DNA in a groove-binding mode that changes the double helical structure of DNA. The binding constant and the number of binding sites calculated from the fluorescence quenching data were 565.718 L mol(-1) and 0.7872, respectively. A molecular modeling study revealed that DMP tends to bind with DNA in the A-T-rich regions of minor groove and that hydrogen bonding and van der Waals forces play main roles in the interaction. This research can help to elucidate the mechanism of DMP toxicity in vivo.
Keywords
Phthalic Acids; phthalic acid; 6O7F7IX66E; 9007-49-2; Index Medicus; dimethyl phthalate; interaction mechanism; Multi-spectroscopic techniques; herring sperm DNA; molecular modeling; Thermodynamics; Animals; Binding Sites -- drug effects; Hydrogen Bonding -- drug effects; Fluorescence; Models, Molecular; Spectrometry, Fluorescence; Circular Dichroism; Spectrophotometry, Ultraviolet; Phthalic Acids -- chemistry; DNA -- drug effects; Phthalic Acids -- toxicity; Fishes -- growth & development; Spermatozoa -- drug effects; Spermatozoa -- growth & development
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch Jan 2016 - July 2016
Pubmed
Excluded: No Primary Data on Health Effects
Not chemical specific
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
WOS
Excluded
Source - Jun 2016 Update (Private)
Pubmed
Source - Dec 2016 Update (Private)
Pubmed
WOS
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity