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Citation
Tags
HERO ID
3860096
Reference Type
Journal Article
Title
Benzyl butyl phthalate decreases myogenic differentiation of endometrial mesenchymal stem/stromal cells through miR-137-mediated regulation of PITX2
Author(s)
Chen, HS; Hsu, CY; Chang, YC; Chuang, HY; Long, CY; Hsieh, TH; Tsai, EM
Year
2017
Is Peer Reviewed?
1
Journal
Scientific Reports
EISSN:
2045-2322
Book Title
Sci Rep.
Volume
7
Issue
1
Page Numbers
186
Language
English
PMID
28298639
DOI
10.1038/s41598-017-00286-6
Web of Science Id
WOS:000425862900001
Abstract
Phthalate, an environmental toxin, has been considered as an endocrine-disrupting chemical. Growing evidence has demonstrated links between endocrine-disrupting chemicals, tissue development, and reproductive physiology, but the mechanisms of gene expression regulation by environmental factors that affect cell differentiation are unclear. Herein, we investigated the effects of butyl benzyl phthalate (BBP) on human endometrial mesenchymal stem/stromal cell (EN-MSC) differentiation and identified a novel signaling pathway. Differentiation of endometrial mesenchymal stem/stromal cells decreased after administration of BBP. We analyzed BBP regulation of gene expression in EN-MSC using cDNA microarrays and Ingenuity Pathway Analysis software to identify affected target genes and their biological functions. PITX2 emerged as a common gene hit from separate screens targeting skeletal and muscular disorders, cell morphology, and tissue development. BBP decreased transcription of PITX2 and elevated expression of the microRNA miR-137, the predicted upstream negative regulator of PITX2. These data indicated that BBP affects PITX2 expression through miR-137 targeting of the 3' untranslated region of PITX2 mRNA. PITX2 down-regulation also decreased MyoD transcript levels in EN-MSC. Our results demonstrate that BBP decreases EN-MSC myogenic differentiation through up-regulation of miR-137, contribute to our understanding of EN-MSC differentiation, and underline the hazardous potential of environmental hormones.
Keywords
3' Untranslated Regions; Cell Differentiation/drug effects; Cells, Cultured; Endometrium/cytology/drug effects/metabolism; Gene Expression Profiling; Gene Expression Regulation/drug effects; Homeodomain Proteins/genetics; Mesenchymal Stem Cells/cytology/drug effects/metabolism; MicroRNAs/genetics; Muscle Development/drug effects; MyoD Protein/genetics; Oligonucleotide Array Sequence Analysis; Phthalic Acids/toxicity; Transcription Factors/genetics; 184787-43-7; YPC4PJX59M
Tags
IRIS
•
BBP (Butyl benzyl phthalate)
Literature Search
Literature Search: February 2017 - July 2017
Pubmed
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jan 2017 - July 2017
Pubmed
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
Excluded
Source - August 2017 Update (Private)
Pubmed
Source - August 2018 Update
WOS
Level 1 Screen - Title & Abstract
Excluded
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