Jump to main content
US EPA
United States Environmental Protection Agency
Search
Search
Main menu
Environmental Topics
Laws & Regulations
About EPA
Health & Environmental Research Online (HERO)
Contact Us
Print
Feedback
Export to File
Search:
This record has one attached file:
Add More Files
Attach File(s):
Display Name for File*:
Save
Citation
Tags
HERO ID
1249832
Reference Type
Journal Article
Title
The endocrine disruptors dibutyl phthalate (DBP) and diethylstilbestrol (DES) influence Leydig cell regeneration following ethane dimethane sulphonate treatment of adult male rats
Author(s)
Heng, K; Anand-Ivell, R; Teerds, K; Ivell, R
Year
2012
Is Peer Reviewed?
Yes
Journal
International Journal of Andrology
ISSN:
0105-6263
EISSN:
1365-2605
Book Title
Int J Androl.
Volume
35
Issue
3
Page Numbers
353-363
Language
English
PMID
22150342
DOI
10.1111/j.1365-2605.2011.01231.x
Web of Science Id
WOS:000304343800015
Abstract
The manner by which endocrine-disrupting xenobiotics, such as phthalates, can induce changes in the development of the male reproductive system still remains largely unknown. Herein, we have explored the application of ethane dimethane sulphonate (EDS) to eliminate adult-type Leydig cells in the mature rat testis, leading to their regeneration from resident stem cells, as a novel system to investigate the effects of dibutyl phthalate (DBP) and diethylstilbestrol (DES) on adult-type Leydig cell differentiation. The advantage of this model is that one can study adult-type Leydig cell differentiation in vivo divorced from the concomitant endocrine development of puberty. In these preliminary studies, we show that both DBP and/or DES, given for 2 or 4 days following EDS application, indeed affect Leydig cell differentiation in the adult testis, largely by increasing early Leydig cell proliferation and possibly thereby delaying early differentiation. In particular, on day 27 post-EDS, a time-point when the differentiation trajectory appears to be most discriminating, we observe that both DBP and/or DES cause a fourfold increase in Leydig cell density, and a significant increase in the expression of the Leydig cell-specific marker transcripts INSL3, LH receptor, Cyp17a1 and Cyp 11a1. In conclusion, both DBP and DES are able to affect adult-type Leydig cells during their differentiation to cause a significant perturbation in their ultimate functional capacity.
Keywords
endocrine disruptors; Leydig cell; rat < animal models; testicular dysgenesis syndrome
Tags
•
Dibutyl Phthalate (DBP)
Database Searches
Pubmed
Web of Science
LitSearch Nov 2012
PubMed
WOS
Merged reference set
LitSearch Dec 2012 - June 2013
Web of Science
Studies with Supporting Data
Mechanistic and genotoxicity studies
Studies with Health Effects Data
Animal toxicology studies
Home
Learn about HERO
Using HERO
Search HERO
Projects in HERO
Risk Assessment
Transparency & Integrity