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Journal Article 
Characterization of estrogenic and androgenic activity of phthalates by the XenoScreen YES/YAS in vitro assay 
Czernych, R; Chraniuk, M; Zagożdżon, P; Wolska, L 
Environmental Toxicology and Pharmacology
ISSN: 1382-6689
EISSN: 1872-7077 
The presented study investigates and compares the estrogenic and androgenic activities of commonly used diesters of phthalic acid (phthalates) using the XenoScreen YES/YAS assay. Phthalates are commonly used plasticizers in polymers dedicated for i.e. food and drug containers. Since phthalates are not chemically bonded to the polymer, they can leach or migrate from the polymer. Therefore, phthalates are identified as contaminants in a variety of consumer products. Investigation of estrogenic and androgenic activities of phthalates (DEP, DBP, BBP, DEHP and DINP) showed no significant effect of tested substances either on hERα or hAR receptors. Phthalates exhibited strong anti-estrogenic (IC50 for BBP=8.66μM, IC50 for DEHP=3.61μM and IC50 for DINP=0.065μM) and anti-androgenic (IC50 for BBP=5.30μM, IC50 for DEHP=2.87μM and IC50 for DINP=0.068μM) activities. 
• BBP (Butyl benzyl phthalate)
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          Literature Search: February 2017 - July 2017
• Dibutyl Phthalate (DBP)
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• Diethyl phthalate (DEP)
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     Excluded: No Primary Data on Health Effects
          Use in sample prep or assay
• Diisononyl Phthalate (DINP)
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               Considered new
• Phthalates – Targeted Search for Epidemiological Studies
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     Source - August 2018 Update
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