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Citation
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HERO ID
3045603
Reference Type
Journal Article
Title
Development of a Highly Specific Fluorescence Immunoassay for Detection of Diisobutyl Phthalate in Edible Oil Samples
Author(s)
Cui, X; Wu, P; Lai, Dan; Zheng, S; Chen, Y; Eremin, SA; Peng, Wei; Zhao, S
Year
2015
Is Peer Reviewed?
Yes
Journal
Journal of Agricultural and Food Chemistry
ISSN:
0021-8561
EISSN:
1520-5118
Book Title
J Agric Food Chem.
Volume
63
Issue
42
Page Numbers
9372-9378
Language
English
PMID
26449794
DOI
10.1021/acs.jafc.5b03922
Web of Science Id
WOS:000363916900019
Abstract
The diisobutyl phthalate (DiBP) hapten containing an amino group was synthesized successfully, and the polyclonal antibody against 4-amino phthalate-bovine serum albumin (BSA) was developed. On the basis of the polyclonal antibody, a rapid and sensitive indirect competitive fluorescence immunoassay (icFIA) has been established to detect DiBP in edible oil samples for the first time. Under the optimized conditions, the quantitative working range of the icFIA was from 10.47 to 357.06 ng/mL (R(2) = 0.991), exhibiting a detection limit of 5.82 ng/mL. In this assay, the specific results showed that other similar phthalates did not significantly interfere with the analysis, with the cross-reactivity less than 1.5%, except for that of DiBAP. Thereafter, DiBP contamination in edible oil samples was detected by icFIA, with the recovery being from 79 to 103%. Furthermore, the reliability of icFIA was validated by gas chromatography-mass spectrometry (GC-MS). Therefore, the developed icFIA is suitable for monitoring DiBP in some edible oil samples.
Keywords
fluorescence immunoassay; phthalate esters; diisobutyl phthalate; edible oil
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jul 2016 - Jan 2017
Prior search overlap
PubMed
Excluded: No Primary Data on Health Effects
Measurement methods
Litsearch June 2015 - Jan 2016
Pubmed
•
Diisobutyl Phthalate (DIBP) Final
Database Searches
December 2015 Update
New for This Search
Pubmed
Web of Science
June 2016 Update
Pubmed
Web of Science
January 2017 Update
No Primary Data on Toxic Effects
Measurement methods
Fate and transport
Exposure levels
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
WOS
Excluded
Source – Dec 2015 Update (Private)
WOS
Source - Jun 2016 Update (Private)
WOS
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