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Citation
Tags
HERO ID
3483280
Reference Type
Journal Article
Title
Analytical method for urinary metabolites as biomarkers for monitoring exposure to phthalates by gas chromatography/mass spectrometry
Author(s)
Yoshida, T
Year
2017
Is Peer Reviewed?
Yes
Journal
Biomedical Chromatography
ISSN:
0269-3879
EISSN:
1099-0801
Volume
31
Issue
7
Page Numbers
E3910-E3910
Language
English
PMID
27925246
DOI
10.1002/bmc.3910
Web of Science Id
WOS:000404968600006
Abstract
Phthalates, widely used as plasticizers, have been detected in indoor air, but there have been few reports on methods of analyzing urinary metabolites as biomarkers to monitor exposure to di-n-pentyl phthalate or di-n-hexyl phthalate. Presented here is a cost-effective and sensitive analytical method for the determination of urinary metabolites of phthalates containing these two compounds. Nine urinary phthalate metabolites were enzymatically hydrolyzed and extracted with toluene: monomethyl phthalate, monoethyl phthalate, monoisobutyl phthalate, mono-n-butyl phthalate, mono-n-pentyl phthalate, mono-n-hexyl phthalate, monocyclohexyl phthalate, monobenzyl phthalate and mono(2-ethyl-5-carboxypentyl) phthalate. After transformation to their tert-butyldimethylsilyl derivatives, they were analyzed by gas chromatography/mass spectrometry in the electron impact ionization mode. The calibration curves for the metabolites were linear at urinary concentrations of up to 30 μg/L, showing that they could be determined accurately and precisely (detection limits 0.1-0.4 μg/L, quantification limits 0.3-1.3 μg/L). The urine samples collected could be stored for up to 1 month at -20°C. The proposed analytical method was used to examine urine samples from seven healthy volunteers. This method should be useful for monitoring phthalate exposure in the general population.
Keywords
Biomarkers/urine; Calibration; Gas Chromatography-Mass Spectrometry/methods; Limit of Detection; Occupational Exposure; Phthalic Acids/urine; Reproducibility of Results; general population; plasticizer; tert-butyldimethylsilyl derivative
Tags
IRIS
•
Dibutyl Phthalate (DBP)
Database Searches
LitSearch Jul 2016 - Jan 2017
Considered new
PubMed
LitSearch Jan 2017 - July 2017
WOS
LitSearch July 2017 - Sept 2018
Prior Search Overlap
WOS
•
Diethyl phthalate (DEP)
Database searches
Jan 2020 update
PubMed
Web of Science
New for this project
Excluded: No Primary Data on Health Effects
Measurement methods
•
Diisobutyl Phthalate (DIBP) Final
Database Searches
January 2017 Update
July 2017 Update
Web of Science
PubMed
No Primary Data on Toxic Effects
Measurement methods
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
Excluded
Source - August 2017 Update (Private)
Pubmed
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