Health & Environmental Research Online (HERO)


Print Feedback Export to File
3981192 
Journal Article 
Biomonitoring of fluoroalkylated substances in Antarctica seabird plasma: Development and validation of a fast and rugged method using on-line concentration liquid chromatography tandem mass spectrometry 
Munoz, G; Labadie, P; Geneste, E; Pardon, P; Tartu, S; Chastel, O; Budzinski, H 
2017 
Yes 
Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778 
1513 
107-117 
English 
We report on a fast, accurate and rugged analytical procedure to determine a wide span of perfluoroalkyl and polyfluoroalkyl substances (PFASs) in seabird plasma. The 26 investigated compounds included perfluoroalkyl carboxylates (C5-C14 PFCAs), perfluoroalkyl sulfonates (C4, C6, C7, C8, C10 PFSAs), perfluorooctane sulfonamide (FOSA) and N-alkyl derivatives (MeFOSA, EtFOSA), N-alkyl perfluorooctane sulfonamido acetic acids (MeFOSAA, EtFOSAA), fluorotelomer sulfonates (4:2 FTSA, 6:2 FTSA, 8:2 FTSA), polyfluoroalkyl phosphate diesters (diPAPs) and perfluorooctane sulfonamide phosphate diester (diSAmPAP). The method described herein requires a reduced sample amount (25μL) and involves rapid and simple sample preparation (protein precipitation with acetonitrile but without acidification) prior to analysis by on-line solid phase extraction (Oasis HLB sorbent) coupled to high performance liquid chromatography negative electrospray ionization tandem mass spectrometry. The optimization was conducted using experimental designs to account for potential interactions between variables. Out of the 26 target analytes, 23 compounds showed excellent accuracy (±25% of the expected values). Intermediate precision and matrix effects remained acceptable for most analytes thanks to efficient internal standardization. A human serum standard reference material (NIST SRM 1957) was included in the validation scheme to evaluate method trueness, which proved satisfactory (│Z-scores│<2 for most compounds). Notwithstanding the small initial sample intake, limits of detection as low as 0.003-0.1ngg(-1) plasma were obtained. This allowed the determination of 11 target PFASs in Antarctic seabird plasma samples. ΣPFASs in Antarctic seabird plasma ranged from 0.37 to 19ngg(-1), with a predominance of PFOS (>54% of ΣPFASs on average). The reduced plasma amount required implies that the present method could also be applied to the analysis of PFASs in the plasma of smaller biological models. 
Alkanesulfonates/analysis/blood; Animals; Antarctic Regions; Calibration; Charadriiformes/blood; Chromatography, High Pressure Liquid/methods; Environmental Monitoring/methods; Fluorocarbons/blood; Limit of Detection; Oceans and Seas; Online Systems; Solid Phase Extraction; Tandem Mass Spectrometry/methods; Time Factors; Antarctica; Biomonitoring; Method validation; On-line solid phase extraction; Polyfluoroalkyl substances; Seabird 
PFAS
• FtS 8:2
     Literature Search
          Pubmed
• ^Per- and Polyfluoroalkyl Substances (PFAS)
     PFOA (335-67-1) and PFOS (1763-23-1)
          Literature Search – Adverse outcome pathway (2015-present)
               Pubmed
• PFNA
     Literature Search
          Pubmed
          Toxline
     PFNA May 2019 Update
          Toxnet
• PFOA (335-67-1) and PFOS (1763-23-1)
     Literature Search – Adverse outcome pathway (2015-present)
          Pubmed
     Screening Results
          Excluded/Not on Topic
     Literature Search Update (2013-2019)
          PubMed
          WOS
• PFOSA
     Literature Search
          Pubmed
     Screening Results
          Excluded/Not on Topic