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Citation
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HERO ID
3230407
Reference Type
Journal Article
Title
Additional oxidized and alkyl chain breakdown metabolites of the plasticizer DINCH in urine after oral dosage to human volunteers
Author(s)
Schütze, A; Otter, R; Modick, H; Langsch, A; Brüning, T; Koch, HM
Year
2016
Is Peer Reviewed?
Yes
Journal
Archives of Toxicology
ISSN:
0340-5761
EISSN:
1432-0738
Publisher
SPRINGER HEIDELBERG
Location
HEIDELBERG
Volume
91
Issue
1
Page Numbers
179-188
Language
English
PMID
26976210
DOI
10.1007/s00204-016-1688-9
Web of Science Id
WOS:000392320700010
URL
http://
://CCC:000392320700010
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Abstract
Hexamoll(®) DINCH(®) (diisononyl-cyclohexane-1,2-dicarboxylate) is a new high molecular weight plasticizer and a non-aromatic phthalate substitute. In this follow-up study, we further investigated the extensive oxidative metabolism of Hexamoll(®) DINCH(®) after oral dosage of 50 mg to three male volunteers (0.552-0.606 mg/kg body weight). Urine samples were consecutively collected over 48 h post-dose. Chemical analysis was carried out by HPLC-MS/MS with labeled internal standards. New metabolites were tentatively identified and quantified via fragmentation analogies and new standard substances. In addition to the five urinary DINCH metabolites previously reported by us, we identified two groups of extensively oxidized metabolites characterized (a) by multiple side chain oxidation and breakdown and (b) by hydroxylation at the cyclohexane ring. The five newly identified carboxylated breakdown metabolites represented in sum 5.12 ± 0.49 % of the applied dose. MCHxCH (cyclohexane-1,2-dicarboxylic acid mono carboxyhexyl ester) was identified as a major metabolite (2.71 ± 0.34 %) and thus represents the second most important specific metabolite of DINCH after OH-MINCH (10.7 ± 2.1 %). Less than 1 % was excreted as ring-hydroxylated metabolites (four metabolites identified). Based upon a new reference standard, we can also update oxo-MINCH to 2.6 % of the applied dose. This follow-up study increases the total amount of the recovered dose from 39.2 to 45.7 % and describes a new major metabolite (MCHxCH) of DINCH that can be used as an additional valuable and specific biomarker to assess DINCH(®) exposure in future human biomonitoring studies.
Tags
•
Dibutyl Phthalate (DBP)
Database Searches
Litsearch Jan 2016 - July 2016
Pubmed
Excluded: No Primary Data on Health Effects
Not chemical specific
•
Phthalates – Targeted Search for Epidemiological Studies
Source – all searches
Pubmed
WOS
Toxnet
Excluded
Source - Jun 2016 Update (Private)
Pubmed
Source - August 2017 Update (Private)
Pubmed
Toxnet
WOS
Source - August 2018 Update
WOS
Toxline
Level 1 Screen - Title & Abstract
Excluded
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