US EPA

1341391 

Journal Article 

[Broad-spectrum analysis of endocrine disruptors in environmental samples] 

Nakada, N; Isobe, T; Nishiyama, H; Okuda, K; Tsutsumi, S; Yamada, J; Kumata, H; Takada, H 

1999 

1 

Bunseki Kagaku / Analysis Chemistry
ISSN: 0525-1931 

Japan Society for Analytical Chemistry 

TOKYO 

48 

6 

535-547 

Japanese 

10.2116/bunsekikagaku.48.535 

WOS:000080930600001 

http:////soc/nacsis.ac.jp/jsac/
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A comprehensive analytical method of endocrine disrupters (i.e., alkylphenols, phthalic acid esters, bisphenol A, polycyclic aromatic hydrocarbons, polychlorinated biphenyls, DDTs) in environmental samples was developed. The method employs Soxhlet-extraction with dichloromethane and two-step-silica-gel and activated-charcoal column chromatography which provides eleven fractions, followed by determination on capillary GC equipped with MSD or ECD. The present method was applied to Tokyo Bay sediments. Most of the target compounds were identified and quantified clearly demonstrating the applicability to various environmental samples. Their concentrations are as follows: 4-t-octylphenol: 10.0 ng/g; 4-t-nonylphenol: 184 ng/g; benzyl n-butyl phthalate: 6.12 ng/g; dicyclohexyl phthalate: 2.56 ng/g; bisphenol A: 6.58 ng/g; benz[a]anthracene: 58.1 ng/g; benzo[a]pyrene: 93.7 ng/g; IUPAC#77 coplanar PCB: 0.82 ng/g; DDT: 0.42 ng/g; DDD: 1.00 ng/g; DDE: 1.37 ng/g. This method provides a powerful analytical tool to investigate a wide range of endocrine disrupters in samples of limited quantity. 

alkylphenols; phthalic acid esters; bisphenol A; polycyclic aromatic hydrocarbons; coplanar PCBs