Phthalates are metabolised by primary thyroid cell cultures but have limited influence on selected thyroid cell functions in vitro | Health & Environmental Research Online (HERO)

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Citation
Tags

HERO ID

3230219

Reference Type

Journal Article

Title

Phthalates are metabolised by primary thyroid cell cultures but have limited influence on selected thyroid cell functions in vitro

Author(s)

Hansen, JF; Brorson, MM; Boas, M; Frederiksen, H; Nielsen, CH; Lindström, ES; Hofman-Bang, J; Hartoft-Nielsen, ML; Frisch, T; Main, KM; Bendtzen, K; Rasmussen, ÅK; Feldt-Rasmussen, U

Year

2016

Is Peer Reviewed?

Yes

Journal

PLoS ONE
EISSN: 1932-6203

Volume

Issue

Page Numbers

e0151192

Language

English

PMID

26985823

DOI

10.1371/journal.pone.0151192

Web of Science Id

WOS:000372580300051

Abstract

Phthalates are plasticisers added to a wide variety of products, resulting in measurable exposure of humans. They are suspected to disrupt the thyroid axis as epidemiological studies suggest an influence on the peripheral thyroid hormone concentration. The mechanism is still unknown as only few in vitro studies within this area exist. The aim of the present study was to investigate the influence of three phthalate diesters (di-ethyl phthalate, di-n-butyl phthalate (DnBP), di-(2-ethylhexyl) phthalate (DEHP)) and two monoesters (mono-n-butyl phthalate and mono-(2-ethylhexyl) phthalate (MEHP)) on the differentiated function of primary human thyroid cell cultures. Also, the kinetics of phthalate metabolism were investigated. DEHP and its monoester, MEHP, both had an inhibitory influence on 3'-5'-cyclic adenosine monophosphate secretion from the cells, and MEHP also on thyroglobulin (Tg) secretion from the cells. Results of the lactate dehydrogenase-measurements indicated that the MEHP-mediated influence was caused by cell death. No influence on gene expression of thyroid specific genes (Tg, thyroid peroxidase, sodium iodine symporter and thyroid stimulating hormone receptor) by any of the investigated diesters could be demonstrated. All phthalate diesters were metabolised to the respective monoester, however with a fall in efficiency for high concentrations of the larger diesters DnBP and DEHP. In conclusion, human thyroid cells were able to metabolise phthalates but this phthalate-exposure did not appear to substantially influence selected functions of these cells.

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