Perfluorinated acid alternatives to trifluoroacetic acid for reversed-phase high-performance liquid chromatography | Health & Environmental Research Online (HERO)

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Citation
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HERO ID

3858375

Reference Type

Journal Article

Title

Perfluorinated acid alternatives to trifluoroacetic acid for reversed-phase high-performance liquid chromatography

Author(s)

Pearson, JD; Mccroskey, MC

Year

1996

Is Peer Reviewed?

Yes

Journal

Journal of Chromatography A
ISSN: 0021-9673
EISSN: 1873-3778

Publisher

Elsevier B.V.

Volume

746

Issue

Page Numbers

277-281

Language

English

PMID

8916558

DOI

10.1016/0021-9673(96)00377-9

Web of Science Id

WOS:A1996VQ49900014

Abstract

Over the past decade trifluoroacetic acid (TFA) has become the ion-pairing agent (IPA) of choice for reversed-phase high-performance liquid chromatography (RP-HPLC) of peptides and proteins. Reagent grade TFA is highly pure, water soluble, transparent at 220 nm and readily volatile. A drawback of this universal appeal is that several alternative perfluorinated carboxylic acids tend to be overlooked when TFA does not work in a particular separation. Examples are given comparing TFA selectivity with those of pentafluoropropionic acid, heptafluorobutyric acid, perfluoropentanoic acid, perfluorohexanoic acid and perfluoroheptanoic acid. We have found that increasing the IPA n-alkyl chain length can resolve sample components that otherwise co-elute in the void volume of TFA-based RP-HPLC. Examples are given for the resolution of an oligoglycine series and enhanced selectivity for a bovine hypothalamic extract.

Keywords

ion-pairing reagents; mobile phase composition; perfluorinated acids; peptides; oligoglycines

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