US EPA

697391 

Journal Article 

Phthalates determination in pharmaceutical formulae used in parenteral nutrition by LC-Phthalates determination in pharmaceutical formulaeES-MS: importance in public health 

Pérez-Feás, C; Barciela-Alonso, M; Sedes-Díaz, A; Bermejo-Barrera, P 

2010 

Yes 

Analytical and Bioanalytical Chemistry
ISSN: 1618-2642
EISSN: 1618-2650 

397 

2 

529-535 

English 

20024534 

10.1007/s00216-009-3337-0 

WOS:000276768400015 

https://search.proquest.com/docview/742685051?accountid=171501
Exit
 

A method for determining a group of phthalate esters in pharmaceutical formulae used in parenteral nutrition samples (with and without vitamins) has been developed. The phthalic acid esters (PAEs) studied were dimethyl phthalate, diethyl phthalate, butyl benzyl phthalate, dibutyl phthalate, di-(2-ethylhexyl) phthalate, and dioctyl phthalate. This group of phthalates was determined by high performance liquid chromatography (HPLC)-electrospray ionization-mass spectrometry, working in positive ion mode. The phthalates analyzed were extracted from the sample using hexane and sodium hydroxide. The hexane was then evaporated, and the compounds were redissolved in acetonitrile. The compounds were separated by HPLC working in gradient mode with acetonitrile-ultrapure water starting from 5% to 75% acetonitrile in 5 min, followed by isocratic elution for 27 min. Standard calibration curves were linear for all the analytes over the concentration range 10-250 microg L(-1). The method was precise (with RSD from 3.3% to 12.9%) and sensitive. The proposed analytical method has been applied to the analysis of these compounds in different pharmaceutical formulae (with different compositions) for parenteral nutrition samples in order to check the presence of phthalates and determine their concentration. 

Phthalates; LC-ES-MS; Parenteral nutrition